1 Select Your Rotor
List of Standard Rotors
| Type | Rotor | Rmin (mm) | Rmax (mm) | Rav (mm) | Angle (°) | Tube ⌀ (mm) | Lsed (mm) |
|---|
2 Properties of the Centrifugation Medium
3 Calculations
Fix two of the three parameters (RCF/RPM, centrifugation time, “cut-off size” of completely sedimented vesicles). The third will be calculated. The proportion of pelleted vesicles in the specified population is calculated automatically.
Centrifugation Protocol
Centrifugation Time
Vesicle Sedimentation Profile
Pelleting Results
| Vesicle diameter | 50 nm | 70 nm | 100 nm | 120 nm | 150 nm |
|---|---|---|---|---|---|
| Portion pelleted | – | – | – | – | – |
4 Sedimentation Profile Plot
5 K-Factor (Clearing Factor) Calculator
The K-factor quantifies rotor pelleting efficiency. Lower K = faster sedimentation. Use it to convert run times between rotors at the same RCF: tnew = told × Knew / Kold
Compare All Rotors
Generate a comparison table showing K-factors and equivalent run times for all preset rotors at the current RCF. Times are scaled relative to the currently selected rotor.
Run Time Conversion
Convert a run time from one rotor to another at the current RCF.
6 Multi-Step Protocol Designer
Design a complete differential centrifugation protocol with multiple steps. Add steps and see cumulative effects on different vesicle populations.